Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: Identification of IL17RB, IL18R1, and IL22RA2 as potential targets of miR-155-5p. IL17RB, IL18R1, and IL22RA2 were identified as potential targets of miR-155-5p by the databases (a); this was confirmed by the dual-luciferase report assay (b). The levels of IL17RB, IL18R1, and IL22RA2 were measured by qRT-PCR (c). *p < 0.05 and **p < 0.01, ANOVA.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Luciferase, Quantitative RT-PCR

Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: Upregulated levels of the inflammatory cytokines in patients with ARDS. The levels of IL17RB, IL18R1, and IL22RA2 in serum samples from patients with ARDS (a). Serum concentrations of IL17RB, IL18R1, and IL22RA2 (b), and IL-1β, IL-6, IL-8, and TNF-α (c) in patients with ARDS were analyzed by ELISA. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Bioengineered

Article Title: MicroRNA-155-5p modulates the progression of acute respiratory distress syndrome by targeting interleukin receptors

doi: 10.1080/21655979.2022.2071020

Figure Lengend Snippet: miR-155-5p inhibited the NF-kB signaling pathway. The expression levels of the inflammatory cytokine receptors (IL17RB, IL18R1, and IL22R2) and NF-kB-related proteins (NF-kB, STAT1, and STAT3) were measured by western blotting analysis with β-actin used as the internal control. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control.

Article Snippet: In brief, LPS reagent was injected into the medium for 24 hours and the relative levels of human IL17RB (EK0785), IL18R1 (EK1260), IL22RA2, IL-1β, IL-6, IL-8, and TNF-α were measured in the treated cells using an ELISA kit (Boster, Wuhan, China) in accordance with the manufacturer’s protocol [ ].

Techniques: Expressing, Western Blot, Control